Bayesian Integrative Region Segmentation in Spatially Resolved Transcriptomic Studies

原文摘要

The spatially resolved transcriptomic study is a recently developed biological experiment that can measure gene expressions and retain spatial information simultaneously, opening a new avenue to characterize fine-grained tissue structures. In this article, we propose a nonparametric Bayesian method named BINRES to carry out the region segmentation for a tissue section by integrating all the three types of data generated during the study—gene expressions, spatial coordinates, and the histology image. BINRES is able to capture more subtle regions than existing statistical partitioning models that only partially make use of the three data modes and is more interpretable than neural-network-based region segmentation approaches. Specifically, due to a nonparametric spatial prior, BINRES does not require a prespecified region number and can learn it automatically. BINRES also combines the image and the gene expressions in the Bayesian consensus clustering framework and thus flexibly adjusts their label alignment contribution weights in a data-adaptive manner. A computationally scalable extension is developed for large-scale studies. Both simulation studies and the real application to three mouse spatial transcriptomic datasets demonstrate that BINRES outperforms the competing methods and easily achieves the uncertainty quantification of the integrative partition. The R package of the proposed method is publicly available at https://github.com/yinqiaoyan/BINRES. Supplementary materials for this article are available online.

摘要来源：OpenAlex 索引摘要（稿件版本未注明）